Gel Electrophoresis Buffers and Diluents
Raybiotech Inc Lysis Buffer, 5 mL, 2X
5 mL of 2X Lysis Buffer. 2X Lysis Buffer should be diluted 2-fold with deionized or distilled water. Supplementation with protease or phosphatase inhibitors is recommended. You may use RayBiotech Protease Inhibitor Cocktail (AA-PI), RayBiotech Phosphatase Inhibitor Cocktail Set I (AA-PHI-I) or Phosphatase Inhibitor Cocktail Set II (AA-PHI-II), or the RayBiotech Phosphatase / Protease Inhibitor Set (AA-PIPHI). Alternatively, you can purchase inhibitor cocktails from another manufacturer and follow their specifications. For the first experiment, we recommend serial dilution testing, starting with a 5-fold dilution, to determine the optimal protein load for the assay. Optimal dilution depends on the abundance of target proteins and should be determined empirically.
Hach Company Hardness 1 Buffer Solution, 1 L
Buffer for hardness and chelant determination by titration, pH 10.1 ± 0.1 at 20C. Used with Hach methods 8213 and 8226. Also used with the Hach Phenols method 8047. 1 liter bottle.
LICOR 10X Orange Loading Dye
10X Orange Loading Dye is used as a loading buffer for DNA gel electrophoresis. This loading dye is not visualized when imaged on Odyssey Imagers. The primary application for 10X Orange Loading Dye is for electrophoretic mobility shift assays (EMSA). When run on a 5% native acrylamide 1X TBE gel, the orange dye migrates with the 20-28 bp DNA fragment.
Zymo Research Corporation 7X Lysis Buffer (Blue) (48 ml)
7X Lysis Buffer (48 ml) (Blue). For use in Zyppy™ Plasmid Purification Kits. Sold individually or as a part of the Zyppy™ plasmid purification product line from Zymo Research.
Quality Biological Inc SDS Protein Gel Loading Solution 5X, 5 mL
Formulated as 5X rather than the traditional 2X stocks, SDS Protein Gel Loading Solution 5X enables a larger volume of sample to be loaded per well. ¿ 5X concentrated protein gel loading buffer ¿ Applications: Simply load the gel sample wells and visualize progress of the electrophoresis run by monitoring the location of the blue dye front. ¿ Lane Marker Sample Buffers may be used in denaturing gels of all kinds and are compatible with coomassie dye and silver staining techniques, as well as Western blotting procedures. ¿ SDS Protein Gel Loading Solution 5X is useful for SDS-PAGE and Western Blotting. ¿ Storage Conditions: 15¿C ¿ 30¿C ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
LaMarKa Chemical Company Methyl Purple Ph7 (Meth) 250Ml
Methyl Purple Indicator Ph 7 (In Methanol)-Bottle 8Oz Plastic
Molecular Dimensions MORPHEUS P550MME-P20K (100 ML)
Protein Crystallography. 100mL: Precipitant Mix 1 (PEG500MME_P20K Mix) from Morpheus Contains a mixture of PEG 500 MME and PEG 20,000 made with ultra-purified water.